3D bioprinting of biomimetic aortic vascular constructs with self-supporting cells

Küçükgül, Can and Özler, Saime Burçe and İnci, İlyas and Karakaş, Hacer Ezgi and Irmak, Ster and Gözüaçık, Devrim and Taralp, Alpay and Koç, Bahattin (2015) 3D bioprinting of biomimetic aortic vascular constructs with self-supporting cells. Biotechnology and Bioengineering, 112 (4). pp. 811-821. ISSN 0006-3592 (Print) 1097-0290 (Online)

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Cardiovascular diseases are the leading cause of deaths throughout the world. Vascular diseases are mostly treated with autografts and blood vessel transplantations. However, traditional grafting methods have several problems including lack of suitable harvest sites, additional surgical costs for harvesting procedure, pain, infection, lack of donors and even no substitutes at all. Recently, tissue engineering and regenerative medicine approaches are used to regenerate damaged or diseased tissues. Most of the tissue engineering investigations have been based on the cell seeding into scaffolds by providing a suitable environment for cell attachment, proliferation and differentiation. Because of the challenges such as difficulties in seeding cells spatially, rejection and inflammation of biomaterials used, the recent tissue engineering studies focus on scaffold-free techniques. In this paper, the development of novel computer aided algorithms and methods are developed for 3D bioprinting of scaffold-free biomimetic macrovascular structures. Computer model mimicking a real human aorta is generated using imaging techniques and the proposed computational algorithms. An optimized three-dimensional bioprinting path planning are developed with the proposed self-supported model. Mouse embryonic fibroblast (MEF) cell aggregates and support structures (hydrogels) are 3D bioprinted layer-by-layer according to the proposed self-supported method to form an aortic tissue construct.
Item Type: Article
Additional Information: Impact Factor: 4.16
Uncontrolled Keywords: 3D bioprinting;scaffold-free tissue engineering;tissue engineering of macrovascular structures;biomimetic modeling
Subjects: Q Science > Q Science (General)
T Technology > T Technology (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng.
Faculty of Engineering and Natural Sciences
Depositing User: Bahattin Koç
Date Deposited: 15 Nov 2014 21:39
Last Modified: 02 Aug 2019 12:04
URI: https://research.sabanciuniv.edu/id/eprint/25094

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