Chemical toxicity detection using quantum dot encoded E. coli cells

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Chouhan, Raghuraj Singh and Kolkar Mohammed, Javed Hussain Niazi and Qureshi, Anjum (2014) Chemical toxicity detection using quantum dot encoded E. coli cells. Sensors and Actuators B: Chemical, 196 . pp. 381-387. ISSN 0925-4005

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Official URL: http://dx.doi.org/10.1016/j.snb.2014.02.027


Bioconjugated quantum dots (QDs) with E. coli cells (bioconjugates) were employed as fluorescent switches that turn-off instantly against any cellular-stress caused by a toxic chemical. Paraquat (PQ), H2O2 and triton X-100 were used as models for assessing their toxicities on bioconjugates. These chemicals interacted on the cell-surfaces where QDs are harbored. The extent of toxicity imposed by chemicals on bioconjugates was successfully probed by (i) real-time fluorescence signals, (ii) visible changes upon UV-light illumination and (iii) scanning electron microscopic (SEM) analysis. Hierarchical cluster analysis using kinetic data of fluorescence and viable cell numbers showed a close relationship between structurally different compounds having similar toxic effects, such as PQ and H2O2, both induced toxicities through generating reactive oxygen species (ROS). In contrast, triton X-100 disrupted the cell–wall integrity and thus showed distinct response due to the loss of cell-bound QDs. Increasing cellular toxicity with chemicals thus followed the order PQ < H2O2 < TX100 confirming the inherent nature of model chemicals to induce cellular toxicity. Our results demonstrated a facile optical strategy that enables rapid and real-time cytotoxicity screening of potentially hazardous chemicals, such as new drugs that lead to ROS generation.

Item Type:Article
Uncontrolled Keywords:E. coli; Quantum dot; Bioconjugation; Fluorescence; Stress inducers; Cytotoxicity
Subjects:T Technology > T Technology (General)
Q Science > QD Chemistry
Q Science > QR Microbiology
ID Code:24040
Deposited By:Javed Kolkar
Deposited On:06 May 2014 15:00
Last Modified:02 Aug 2019 10:00

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