Çimen, Atike Nur and Çelebi Torabfam, Gizem and Tok, Yesim Tuyji and Yucebag, Ebru and Arslan, Neşe and Saribal, Devrim and Esken, Gülen and Dogan, Özlem and Kuskucu, Mert Ahmet and Mete, Bilgul and Aygun, Gokhan and Tabak, Fehmi and Can, Füsun and Ergonul, Onder and Midilli, Kenan and Kutlu, Özlem and Çetinel, Sibel (2024) Development of pseudotyped VSV-SARS-CoV-2 spike variants for the assessment of neutralizing antibodies. Bioanalysis, 16 (21-22). pp. 1167-1177. ISSN 1757-6180 (Print) 1757-6199 (Online)
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Official URL: https://dx.doi.org/10.1080/17576180.2024.2411920
Abstract
Aim: Serological studies with pseudotyped viruses offer a safer alternative to live SARS-CoV-2 in evaluating neutralizing antibodies, enabling research in standard labs. Methods: The SARS-CoV-2 Spike pseudotyped vesicular stomatitis virus (VSV) pseudoviruses were generated using Spike of Wuhan strain and two variants (B.1.1.7, B.1.351) and utilized to evaluate the serum neutralizing activity of human plasma samples of vaccinated (n = 13) and healthy people (n = 2) compared with a plaque assay with authentic virus. Results: Neutralizing titer of convalescent plasma resulted with a good correlation (R2 = 0.7). Conclusion: We evaluated a safe and reliable pseudotyped virus system that effectively mimics authentic virus and correlates well with traditional assays. The developed system allows easier testing of variants and has the potential to improve vaccine development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | convalescent plasma; neutralization assay; pseudovirus assay; Spike variants; VSV-SARS-CoV-2 |
| Divisions: | Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng. Faculty of Engineering and Natural Sciences Sabancı University Nanotechnology Research and Application Center |
| Depositing User: | Sibel Çetinel |
| Date Deposited: | 20 Dec 2024 15:54 |
| Last Modified: | 20 Dec 2024 15:54 |
| URI: | https://research.sabanciuniv.edu/id/eprint/50543 |
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