Design of poly(vinyl pyrrolidone) and poly(ethylene glycol) microneedle arrays for delivering glycosaminoglycan, chondroitin sulfate, and hyaluronic acid

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Choupani, Andisheh and Temuçin, Elif Şevval and Çiftci Dede, Eda and Bakan Mısırlıoğlu, Feray and Çamiç, Büşra Tuğba and Özkoç, Güralp and Sezen, Meltem and Korkusuz, Petek and Korkusuz, Feza and Bediz, Bekir (2024) Design of poly(vinyl pyrrolidone) and poly(ethylene glycol) microneedle arrays for delivering glycosaminoglycan, chondroitin sulfate, and hyaluronic acid. Journal of Biomaterials Science, Polymer Edition . ISSN 0920-5063 (Print) 1568-5624 (Online) Published Online First https://dx.doi.org/10.1080/09205063.2024.2392914

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Abstract

Osteoarthritis (OA) is a prevalent joint disorder characterized by cartilage and bone degradation. Medical therapies like glucosaminoglycan (GAG), chondroitin sulfate (CS), and hyaluronic acid (HA) aim to preserve joint function and reduce inflammation but may cause side effects when administered orally or via injection. Microneedle arrays (MNAs) offer a localized drug delivery method that reduces side effects. Thus, this study aims to demonstrate the feasibility of delivering GAG, CS, and HA using microneedles in vitro. An optimal needle geometry is crucial for the successful application of MNA. To address this, here we employ a multi-objective optimization framework using the non-dominated sorting genetic algorithm II (NSGA-II) to determine the ideal MNA design, focusing on preventing needle failure. Then, a three-step fabrication approach is followed to fabricate the MNAs. First, the master (male) molds are fabricated from poly(methyl methacrylate) using mechanical micromachining based on optimized needle geometry. Second, a micro-molding with Polydimethylsiloxane (PDMS) is used for the fabrication of production (female) molds. In the last step, the MNAs were fabricated by microcasting the hydrogels using the production molds. Light microscopy (LIMI) confirms the accuracy of the MNAs manufactured, and in vitro skin insertion tests demonstrate failure-free needle insertion. Subsequently, we confirmed the biocompatibility of MNAs by evaluating their impact on the L929 fibroblast cell line, human chondrocytes, and osteoblasts.
Item Type: Article
Uncontrolled Keywords: chondroitin sulfate; glycosaminoglycan; hyaluronic acid; Microneedle; microneedle array; needle geometry optimization
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Mechatronics
Faculty of Engineering and Natural Sciences
Sabancı University Nanotechnology Research and Application Center
Integrated Manufacturing Technologies Research and Application Center
Depositing User: Bekir Bediz
Date Deposited: 28 Sep 2024 22:46
Last Modified: 28 Sep 2024 22:46
URI: https://research.sabanciuniv.edu/id/eprint/50239

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