Functional Neurons From Human Bone Marrow Derived Mesenchymal Stem Cells

Kuş, Eda (2023) Functional Neurons From Human Bone Marrow Derived Mesenchymal Stem Cells. [Thesis]

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Study of neurological pathology mechanisms is essential to understand and create therapies of neurological disorders. However, due to the human brain’s complexity and inaccessibility, research is limited. Additionally, there isn’t a well established neuronal cell line available and animal studies fail to reflect physiology of human brain disorders. To address these limitations, stem cell technologies are being developed to create more accurate disease models. Mesenchymal stem cells have emerged as a preferred option for neuronal differentiation due to their multipotency, accessibility, and low immune response properties. Various methods have been employed to differentiate mesenchymal stem cells into neurons, but it is crucial to ensure that the resulting neurons possess functional characteristics resembling a complete neuronal unit within the model. In this study, we describe the process of differentiating human bone marrow-derived mesenchymal stem cells into neurons using a specific differentiation medium supported with extracellular matrix components. We evaluate the functionality of these differentiated neurons using multielectrode arrays, providing possible valuable insights for future research in this field.
Item Type: Thesis
Uncontrolled Keywords: mesenchymal stem cells, neuronal differentiation, multi electrode arrays, graphene nanoplatelets. -- mezenkimal kök hücre, nöronal farklılaşma, çok elektrotlu diziler, grafen nanoplatelet.
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng.
Faculty of Engineering and Natural Sciences
Depositing User: Dila Günay
Date Deposited: 20 Dec 2023 10:56
Last Modified: 20 Dec 2023 10:56

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