Multifunctional tetracycline-loaded silica-coated core-shell magnetic nanoparticles: antibacterial, antibiofilm, and cytotoxic activities

Mazraeh, Maryam and Eshrati Yeganeh, Faten and Yousefi, Mohammad and Baniyaghoob, Sahar and Farasati Far, Bahareh and Akbarzadeh, Iman and Bigham, Ashkan and Ashrafizadeh, Milad and Rabiee, Navid and Makvandi, Pooyan and Saeb, Mohammad Reza (2022) Multifunctional tetracycline-loaded silica-coated core-shell magnetic nanoparticles: antibacterial, antibiofilm, and cytotoxic activities. ACS Applied Bio Materials, 5 (4). pp. 1731-1743. ISSN 2576-6422

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Abstract

In the current study, the physicochemical and biological properties of tetracycline-loaded core-shell nanoparticles (Tet/Ni0.5Co0.5Fe2O4/SiO2 and Tet/CoFe2O4/SiO2) were investigated. The antibacterial activity of nanoparticles alone and in combination with tetracycline was investigated against a number of Gram-positive and Gram-negative bacteria for determining minimum inhibitory concentration (MIC) values. The MIC of Tet/Ni0.5Co0.5Fe2O4/SiO2 nanoparticles turned out to be significantly higher than that of Tet/CoFe2O4/SiO2 nanoparticles. Furthermore, Tet/Ni0.5Co0.5Fe2O4/SiO2 nanoparticles exhibited potent antibiofilm activity against pathogenic bacteria compared to Tet/CoFe2O4/SiO2 nanoparticles. The drug delivery potential of both carriers was assessed in vitro up to 124 h at different pH levels and it was found that the drug release rate was increased in acidic conditions. The cytotoxicity of nanoparticles was evaluated against a skin cancer cell line (melanoma A375) and a normal cell line (HFF). Our findings showed that Tet/Ni0.5Co0.5Fe2O4/SiO2 had greater cytotoxicity than CoFe2O4/SiO2 against the A375 cell line, whereas both synthesized nanoparticles had no significant cytotoxic effects on the normal cell line. Nonetheless, the biocompatibility of nanoparticles was assessed in vivo and the interaction of nanoparticles with the kidney was scrutinized up to 14 days. The overall results of the present study implied that the synthesized multifunctional magnetic nanoparticles with drug delivery potential, anticancer activity, and antibacterial activity are promising for biomedical applications.
Item Type: Article
Uncontrolled Keywords: antibacterial; antibiofilm; antibiotic; biofilm gene; cytotoxicity
Divisions: Faculty of Engineering and Natural Sciences
Depositing User: Milad Ashrafizadeh
Date Deposited: 22 Aug 2022 20:11
Last Modified: 22 Aug 2022 20:12
URI: https://research.sabanciuniv.edu/id/eprint/44135

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