Quantitative investigation into the influence of intravenous fluids on human immune and cancer cell lines

Karamahmutoğlu, Hande and Altay, Alara and Vural Kaymaz, Sümeyra and Elitaş, Meltem (2020) Quantitative investigation into the influence of intravenous fluids on human immune and cancer cell lines. Scientific Reports, 10 . ISSN 2045-2322

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The effect of intravenous fluids (IVF) has been investigated clinically through the assessment of post-treatment reactions. However, the responses to IVF vary from patient-to-patient. It is important to understand the response of IVF treatment to be able to provide optimal IVF care. Herein, we investigated the impact of commonly used IVFs, Dextrose, NaCl and Ringer on different human cancer (HepG2 (liver hepatocellular carcinoma) and MCF7 (breast adenocarcinoma)) and immune cell lines (U937 (lymphoma) monocyte and macrophages). The effect of IVF exposure on single cells was characterized using hemocytometer, fluorescence microscopy and flow cytometry. Quantitative data on the viability and morphology of the cells were obtained. Our results emphasize that different IVFs demonstrate important differences in how they influence distinct cell lines. Particularly, we observed that the lactated ringer and dextrose solutions altered the viability and nuclear size of cancer and immune cells differently. Our findings present valuable information to the knowledge of cellular-level IVF effects for further investigations in IVF usage on diverse patient populations and support the importance and necessity of developing optimal diluents not only for drug stability but also for patient benefits.
Item Type: Article
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Mechatronics
Sabancı University Nanotechnology Research and Application Center
Faculty of Engineering and Natural Sciences
Depositing User: Meltem Elitaş
Date Deposited: 19 Jul 2020 10:43
Last Modified: 02 Aug 2023 12:27
URI: https://research.sabanciuniv.edu/id/eprint/40033

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