Efficient embryogenic callus from filaments with anther in thermopsis turcica

Tekdal, Dilek and Çetiner, Selim (2014) Efficient embryogenic callus from filaments with anther in thermopsis turcica. Turkish Journal of Agricultural and Natural Sciences (SI), 1 . pp. 1242-1246. ISSN 2148-3647

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Thermopsis turcica is an endemic endangered species. The establishment of an improved micropropagation method is very important to preserve this species in vitro conditions. For this reason, the effects of different media and temperature pretreatments on the stamens of T. turcica planted at Nezahat Gokyigit Botanical Garden in Istanbul were investigated. T. turcica filaments with anther were excised and cultivated in vitro with Murashige and Skoog’s basal media supplemented with Naphthaleneacetic acid (4 mg L- 1), Benzylaminopurine (0.1 and 0.5 mg L-1), and AgNO3 (15 mg L-1) at 4, 28 and 37°C for a week under complete darkness. Moreover, to determine the effects of activated charcoal on regeneration, a medium containing 0.25% activated charcoal was also used. A week after culture initiation, all samples were then incubated at 28°C for a month under a 16 h photoperiod. The most promising callus formation was observed in filaments cultured on the media with 0.1 mg L-1 Benzylaminopurine (100%) pretreated at 37°C a week after culture initiation; however, the development of embryos from callus could also be visualized from the filaments cultured on the medium with 0.1 mg L-1 Benzylaminopurine (50%) pretreated at 4°C. None of the activated charcoal treatments yielded positive results regarding callus formation. Instead they negatively affected callus formation in the mixture media. Furthermore, control groups that were incubated on MS medium free from growth regulators did not show any growth. According to a literature review on T. turcica, no stamen culture has been performed to date.
Item Type: Article
Uncontrolled Keywords: Callus, embryo, filament, Thermopsis turcica
Subjects: S Agriculture > SB Plant culture > SB403-450.87 Flowers and flower culture. Ornamental plants
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng.
Faculty of Engineering and Natural Sciences
Depositing User: Dilek Tekdal
Date Deposited: 20 May 2015 11:34
Last Modified: 02 Aug 2019 16:17
URI: https://research.sabanciuniv.edu/id/eprint/26917

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