Efficient embryogenic callus from filaments with anther in thermopsis turcica

Official URL: http://dergipark.ulakbim.gov.tr/ttdb/article/view/5000090968/5000084371

Thermopsis turcica is an endemic endangered species. The establishment of an improved micropropagation method is very important to preserve this species in vitro conditions. For this reason, the effects of different media and temperature pretreatments on the stamens of T. turcica planted at Nezahat Gokyigit Botanical Garden in Istanbul were investigated. T. turcica filaments with anther were excised and cultivated in vitro with Murashige and Skoog’s basal media supplemented with Naphthaleneacetic acid (4 mg L- 1), Benzylaminopurine (0.1 and 0.5 mg L-1), and AgNO3 (15 mg L-1) at 4, 28 and 37°C for a week under complete darkness. Moreover, to determine the effects of activated charcoal on regeneration, a medium containing 0.25% activated charcoal was also used. A week after culture initiation, all samples were then incubated at 28°C for a month under a 16 h photoperiod. The most promising callus formation was observed in filaments cultured on the media with 0.1 mg L-1 Benzylaminopurine (100%) pretreated at 37°C a week after culture initiation; however, the development of embryos from callus could also be visualized from the filaments cultured on the medium with 0.1 mg L-1 Benzylaminopurine (50%) pretreated at 4°C. None of the activated charcoal treatments yielded positive results regarding callus formation. Instead they negatively affected callus formation in the mixture media. Furthermore, control groups that were incubated on MS medium free from growth regulators did not show any growth. According to a literature review on T. turcica, no stamen culture has been performed to date.