In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Official URL: http://dx.doi.org/10.2478/s11756-014-0383-7

This plant tissue study of micropropagation identifies the selective medium saving for rapid propagation in cultivated Thermopsis turcica, an endangered germplasm of the family Fabaceae. The aim is to obtain the optimum growth medium of T. turcica by enabling the in vitro propagation of this endemic. In this study, the leaves and stems of T. turcica were cultured on a Murashige and Skoog's medium supplemented with various concentrations (0.5, 1.0 and 2.0 mg L-1 1-Naphthaleneacetic acid) of auxin and (0.2 and 0.5 mg L-1 Zeatin) (1.0 and 2.0 mg L-1 Benzylaminopurine) of cytokinins. Previous research focused on the regeneration from the seed of T. turcica Eber population; we concentrated upon the regeneration of different plant parts (leaf and stem) of T. turcica Aksehir population. In addition, according to the literature on T. turcica that to date the effects of Zeatin on the regeneration has not been performed. The most promising regeneration and growth were obtained from leaf explants cultured on the media with 2.0 mg L-1 1-Naphthaleneacetic acid and 0.5 mg L(-1)Zeatin (93.3%). The regenerated plantles were rooted on the media containing 2.0 mg L-1 Indole-3-butyric acid. Rooted plantlets were transplanted into potting of sterilized soil. The present study reports on the sufficient in vitro regeneration protocol through organogenesis in T. turcica. The findings presented here have implications for in vitro protection and use of this endemic endangered species in further biotechnological research.