Sandwichtype, antibody microarrays for detection and quantification of cardiovascular risk markers

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Official URL: http://www.sciencedirect.com/science?_ob=ArticleListURL&_method=list&_ArticleListID=552448174&_sort=d&view=c&_acct=C000038658&_version=1&_urlVersion=0&_userid=691258&md5=48fc99ae66f307a3ac7e18bca71b421a

In this paper, we have analyzed the capability and reliability of sandwich-type antibody microarrays for the detection quantification of cardiovascular risk markers. We used optical scanner system for the detection of fluorescent-labeled, sandwiched-structured antibodies on the chemically modified glass substrates. With the process and structure presented in this study, baseline and elevated levels of C-Reactive Protein, Myoglobin, TNF-α, Serum Amyloid A proteins, known cardiovascular risk markers in human serum, can be detected and quantified. This study is the first study of its kind, utilizing sandwich-type arrays for the detection and quantification of multiple-cardiovascular risk markers in human serum. We have also presented, in this study, that anti-fatty-acid binding protein antibodies, used as capture molecules, cross reacts with all detection antibodies on the microarray platform, generating noise signals, interfering the detection and quantification of markers. Finally, we have compared the results of our study with the performance of commercially available ELISA test-kits that presenting the advantages such as being able to detect and quantify multiple markers at once and also better dynamic ranges over some of the commercial ELISA test-kits.