Through the looking glass: real-time imaging in brachypodium roots and osmotic stress analysis

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Khan, Zaeema and Karamahmutoğlu, Hande and Elitaş, Meltem and Yüce, Meral and Budak, Hikmet (2019) Through the looking glass: real-time imaging in brachypodium roots and osmotic stress analysis. Plants, 8 (1). ISSN 2223-7747

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Official URL: http://dx.doi.org/10.3390/plants8010014


To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.

Item Type:Article
Uncontrolled Keywords:Brachypodium; neutral red; root; Casparian bands; PEG-6000; osmotic stress; real-time imaging; PDMS
Subjects:S Agriculture > S Agriculture (General)
T Technology > T Technology (General)
ID Code:38647
Deposited By:Meltem Elitaş
Deposited On:22 Aug 2019 16:00
Last Modified:08 May 2020 14:40

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