Dispersion quality of amine functionalized multiwall carbon nanotubes plays critical roles in polymerase chain reaction enhancement

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Yüce, Meral and Budak, Hikmet (2014) Dispersion quality of amine functionalized multiwall carbon nanotubes plays critical roles in polymerase chain reaction enhancement. Journal of Nanoparticle Research, 16 (12). ISSN 1388-0764 (Print) 1572-896X (Online)

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Official URL: http://dx.doi.org/10.1007/s11051-014-2768-5


Impact of dispersion quality of NH2-MWCNTs (13-18 nm in diameter with a length between 1 and 12 mu m, >99 % purity) in the amplification efficiency of a random DNA oligonucleotide library (96 bp) was investigated. Amplification yield in the presence of non-filtered NH2-MWCNT dispersion, filtered NH2-MWCNT dispersion and surface-attached NH2-MWCNTs was explored, and physical interactions between NH2-MWCNTs and major PCR reagents including DNA template, wild type Taq DNA polymerase enzyme and primers were determined using high resolution polyacrylamide gel electrophoresis, dynamic light scattering, UV-Vis-NIR spectroscopy and scanning electron microscopy techniques. The results revealed that presence of NH2-MWCNT dispersion which was sonicated, centrifuged and filtered, enhanced the total PCR efficiency up to 70 % while the presence of NH2-MWCNT only centrifuged after sonication, inhibited the reaction significantly at similar concentrations. Furthermore, the NH2-MWCNTs coupled covalently onto magnetic microspheres, contributed for the specificity enhancement whilst decreasing the amplification efficiency by 30 % at the maximum concentration, which suggests a removable enhancement system for sensitive applications. On the other hand, the relative hydrodynamic size distribution measurements displayed a clear difference between the filtered NH2 and non-filtered NH2-MWCNT water dispersions, which justifies the inhibition of the amplification by the non-filtered NH2-MWCNTs containing big agglomerates and bundles. Finally, we demonstrated that major PCR components adsorb onto the NH2-MWCNTs with diverse affinities, and maintain their functions after adsorption, which provides a good framework to further develop tunable NH2-MWCNT-carriers to be utilized in various nanobiotechnology and material science applications.

Item Type:Article
Additional Information:Article Number: 2768
Uncontrolled Keywords:Polymerase chain reaction; Nano PCR; Amine functionalized multiwall carbon nanotubes; Random DNA oligonucleotide library; Magnetic beads
Subjects:Q Science > Q Science (General)
ID Code:27574
Deposited By:Meral Yüce
Deposited On:20 Nov 2015 11:13
Last Modified:23 Aug 2019 11:27

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