Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules

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Ünal, Hayriye and Kolkar Mohammed, Javed Hussain Niazi (2013) Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules. Journal of Materials Chemistry B, 1 (14). pp. 1894-1902. ISSN 2050-750X

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Official URL: http://dx.doi.org/10.1039/c3tb00563a


Carbon nanotube (CNT) decorated magnetic microspheres were fabricated to develop a multimodal platform that utilizes non-covalent molecular interactions of CNTs to magnetically separate biomolecules. Hybrid CNT-microspheres prepared by a feasible method reported herein had a well-defined structure as characterized by Raman spectroscopy and scanning electron microscopy. Binding interactions of resulting magnetic CNT-microspheres with DNA oligonucleotides were studied to demonstrate that single stranded DNA (ssDNA) in a solution can be effectively recovered by magnetic CNT-microspheres through strong physical wrapping of DNA around CNTs' walls. The magnetic character of these CNT-microspheres combined with their capability to bind other molecules including DNA allows their use as an affinity matrix that can be utilized in affinity separation of biomolecules, and also as a platform to monitor non-covalent binding interactions of CNTs with other biomolecules. As a proof of concept, we report on the use of these CNT-microspheres in in vitro selection of ssDNA aptamers against carcinoembryonic antigen (CEA), a cancer biomarker, by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). ssDNA aptamer candidates that have strong affinity towards CEA were successfully separated magnetically from a pool of ssDNA (1014 molecules). Our results demonstrate that CNT-microspheres can serve as strong tools for affinity separation methodologies and can be utilized for various affinity pairs in solution.

Item Type:Article
Subjects:T Technology > T Technology (General)
ID Code:21603
Deposited By:Javed Kolkar
Deposited On:21 Jun 2013 10:34
Last Modified:31 Jul 2019 16:46

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