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Single-cell mechanophenotyping in microfluidics to evaluate behavior of U87 glioma cells

Şengül, Esra and Elitaş, Meltem (2020) Single-cell mechanophenotyping in microfluidics to evaluate behavior of U87 glioma cells. Micromachines, 11 (9). ISSN 2072-666X

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Official URL: http://dx.doi.org/10.3390/mi11090845

Abstract

Integration of microfabricated, single-cell resolution and traditional, population-level biological assays will be the future of modern techniques in biology that will enroll in evolution of biology into a precision scientific discipline. In this study, we developed a microfabricated cell culture platform to investigate indirect influence of macrophages on glioma cell behavior. We quantified proliferation, morphology, motility, migration, and deformation properties of glioma cells at single-cell level and compared these results with population-level data. Our results showed that glioma cells obtained slightly slower proliferation, higher motility and extremely significant deformation capability when cultured with 50% regular growth medium and 50% macrophage-depleted medium. When the expression levels of E-cadherin and Vimentin proteins were measured, it was verified that observed mechanophenotypic alterations in glioma cells were not due to epithelium to mesenchymal transition. Our results were consistent with previously reported enormous heterogeneity of U87 glioma cell line. Herein, for the first time, we quantified the change of deformation indexes of U87 glioma cells using microfluidic devices for single-cells analysis.

Item Type:Article
Uncontrolled Keywords:glioblastoma; mechanophenotyping; deformation; migration; Label-free; single-cell
Subjects:Q Science > Q Science (General)
T Technology > T Technology (General) > T174.7 Nanotechnology
ID Code:40603
Deposited By:Meltem Elitaş
Deposited On:13 Sep 2020 10:42
Last Modified:13 Sep 2020 10:42

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