Barakat, Sarah and Yang, Fan and Yelkenci, Hayriye Ecem and Kök, Kıvanç and Mann, Giovanni E. and Eroğlu, Emrah (2025) Proteomic data and drug implications for cerebral microvascular endothelial cells under varying oxygen levels. Scientific Data, 12 (1). ISSN 2052-4463
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Official URL: https://dx.doi.org/10.1038/s41597-025-05160-z
Abstract
Hyperoxia in standard cell cultures (18 kPa O2) imposes cellular oxidative stress, potentially skewing research and drug screening outcomes. Cerebral microvascular endothelial cells (hCMEC/D3) experience no more than 7 kPa O2in vivo. In this study, hCMEC/D3 cells were adapted to 5 kPa O2 for 5 days to optimize an in vitro physiological cell culture model. Using a SYNAPT G2-Si mass spectrometer, we compared the proteomic profiles of cells cultured under 5 kPa versus 18 kPa O2. A substantial proteomic shift under hyperoxia highlighted the strong impact of oxygen levels on protein expression. We further investigated the effect of oxygen levels on drug screening using sulforaphane (SFN), an inducer of NRF2-regulated antioxidant defense genes. SFN induced more pronounced changes in proteomic profiles under 18 kPa O2 compared to 5 kPa, indicating oxygen-dependent cellular drug responses. This dataset offers a valuable resource for analyzing oxygen-sensitive proteomic changes. Comparative studies using different drugs or cell types could further elucidate oxygen-dependent signaling and inform the development of therapies aligned with physiological oxygen levels.
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| Additional Information: | This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/. |
| Divisions: | Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng. Faculty of Engineering and Natural Sciences |
| Depositing User: | Emrah Eroğlu |
| Date Deposited: | 29 Aug 2025 15:29 |
| Last Modified: | 20 Oct 2026 16:41 |
| URI: | https://research.sabanciuniv.edu/id/eprint/52010 |
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