The effect of geometry on cell activity for crosstalk avoidance using a stimulated brain co-culture system on a multielectrode array

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Delipınar, Tuğçe (2022) The effect of geometry on cell activity for crosstalk avoidance using a stimulated brain co-culture system on a multielectrode array. [Thesis]

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Abstract

Unidirectional and independent growth of axons in in-vitro brain co-culture systems is crucial to establish a signal transmission platform which is capable of transmitting independent signals from source to target with minimum to no cross-talk and channel interference. In this thesis, primary thalamus cells were cultured on multielectrode arrays, aiming to mimic interfacing the LGN through enhanced synapse formation between the seeded source and target cells. Electrical stimulation and baseline activity recordings were analyzed to track cell activity and cross-talk. Axon-guiding microstructures of various designs were tested to observe axon growth directionality under the effect of different design parameters. According to the obtained results, rescue loops and merging structures performed well in guiding the axons in correct directions and changes in channel width influenced the design performance. With the approach in this thesis, it is possible to overcome the hurdles related to the low spatiotemporal resolution of DBS electrodes by carrying the electrodes outside of the brain which gives flexibility to define specific electrode positions and neuron insulation for cross-talk prevention.
Item Type: Thesis
Uncontrolled Keywords: brain co-culture systems. -- in vitro. -- electrical stimulation. -- spike detection. -- MEA, cross-talk. -- unidirectional axon growth. -- axon-guiding microstructures. -- beyin ortak kültür sistemleri. -- in vitro. -- elektriksel stimülasyon. -- yükselen sinyal algılama. -- çapraz konuşma. -- tek yönlü akson büyümesi. -- akson yönlendirici mikro yapılar.
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA401-492 Materials of engineering and construction. Mechanics of materials
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Materials Science & Eng.
Faculty of Engineering and Natural Sciences
Depositing User: Dila Günay
Date Deposited: 04 Jul 2023 16:19
Last Modified: 13 Nov 2023 14:57
URI: https://research.sabanciuniv.edu/id/eprint/47419

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