On the application of hydrodynamic cavitation on a chip in cellular injury and drug delivery

Namlı, İlayda and Karavelioglu, Zeynep and Seyedmirzaei Sarraf, Seyedali and Sheibani Aghdam, Araz and Varol, Rahmetullah and Yilmaz, Abdurrahim and Şahin, Sevilay Burcu and Özogul, Beyzanur and Bozkaya, Dila Naz and Acar, Havva Funda and Uvet, Huseyin and Çetinel, Sibel and Kutlu, Özlem and Ghorbani, Morteza and Koşar, Ali (2023) On the application of hydrodynamic cavitation on a chip in cellular injury and drug delivery. Lab On a Chip, 23 (11). pp. 2640-2653. ISSN 1473-0197 (Print) 1473-0189 (Online)

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Hydrodynamic cavitation (HC) is a phase change phenomenon, where energy release in a fluid occurs upon the collapse of bubbles, which form due to the low local pressures. During recent years, due to advances in lab-on-a-chip technologies, HC-on-a-chip (HCOC) and its potential applications have attracted considerable interest. Microfluidic devices enable the performance of controlled experiments by enabling spatial control over the cavitation process and by precisely monitoring its evolution. In this study, we propose the adjunctive use of HC to induce distinct zones of cellular injury and enhance the anticancer efficacy of Doxorubicin (DOX). HC caused different regions (lysis, necrosis, permeabilization, and unaffected regions) upon exposure of different cancer and normal cells to HC. Moreover, HC was also applied to the confluent cell monolayer following the DOX treatment. Here, it was shown that the combination of DOX and HC exhibited a more pronounced anticancer activity on cancer cells than DOX alone. The effect of HC on cell permeabilization was also proven by using carbon dots (CDs). Finally, the cell stiffness parameter, which was associated with cell proliferation, migration and metastasis, was investigated with the use of cancer cells and normal cells under HC exposure. The HCOC offers the advantage of creating well-defined zones of bio-responses upon HC exposure simultaneously within minutes, achieving cell lysis and molecular delivery through permeabilization by providing spatial control. In conclusion, micro scale hydrodynamic cavitation proposes a promising alternative to be used to increase the therapeutic efficacy of anticancer drugs.
Item Type: Article
Divisions: Faculty of Engineering and Natural Sciences
Sabancı University Nanotechnology Research and Application Center
Depositing User: Sibel Çetinel
Date Deposited: 06 Aug 2023 18:19
Last Modified: 06 Aug 2023 18:19
URI: https://research.sabanciuniv.edu/id/eprint/47318

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