Identification of the targets of the human transcription factor patz1

Official URL: https://risc01.sabanciuniv.edu/record=b2767367_(Table of contents)

POZ-AT hook-zinc finger protein 1 (PATZ1) is a ubiquitously expressed N-terminal BTB domain containing transcription factor which has a C2H2 type zinc finger DNA binding domain at its C-terminal. According to the recent findings PATZ1 acts both as a suppressor and an activator in a context dependent manner. Even though regulatory role of Patz1 is associated with various cancers, such as Ewing’s Sarcoma and prostate cancer, the target genes of PATZ1 are not known. As a transcription factor remains controversial due to lack of genomic studies. We identified some of the genes that are bound and regulated by the PATZ1 transcription factor in the HCT116 colon cancer cell line by using techniques such as CRISPR/Cas9 genome editing tool, RNA-seq and CHIPseq data analysis. We showed that PATZ1 binds to the promoters of its target genes and regulates their expression by both activating and suppressing these targets. For the first time in literature, we identified transcriptional targets of PATZ1 in HCT116 human colon cancer cell line.