An injectable peptide hydrogel for reconstruction of the human trabecular meshwork

Waduthanthri, Kosala D. and He, Yuan and Montemagno, Carlo and Çetinel, Sibel (2019) An injectable peptide hydrogel for reconstruction of the human trabecular meshwork. Acta Biomaterialia, 100 . pp. 244-254. ISSN 1742-7061 (Print) 1878-7568 (Online)

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Glaucoma is a leading cause of irreversible blindness worldwide. Current treatments of glaucoma involve lowering the IOP by means of decreasing aqueous humor production or increasing non-trabecular aqueous humor outflow with the help of IOP-lowering eye drops, nanotechnology enabled glaucoma drainage implants, and trabeculectomy. However, there is currently no effective and permanent cure for this disease. In order to investigate new therapeutic strategies, three dimensional (3D) biomimetic trabecular meshwork (TM) models are in demand. Therefore, we adapted MAX8B, a peptide hydrogel system to bioengineer a 3D trabecular meshwork scaffold. We assessed mechanical and bio-instructive properties of this engineered tissue matrix by using rheological analysis, 3D cell culture and imaging techniques. The scaffold material exhibited shear-thinning ability and biocompatibility for proper hTM growth and proliferation indicating a potential utilization as an injectable implant. Additionally, by using a perfusion system, MAX8B scaffold was tested as an in vitro platform for investigating the effect of Dexamethasone (Dex) on trabecular meshwork outflow facility. The physiological response of hTM cells within the scaffold to Dex treatment clearly supported the effectiveness of this 3D model as a drug-testing platform, which can accelerate discovery of new therapeutic targets for glaucoma. Statement of significance: Artificial 3D-TM (3-dimentional Trabecular Meshwork) developed here with hTM (human TM) cells seeded on peptide-hydrogel scaffolds exhibits the mechanical strength and physiological properties mimicking the native TM tissue. Besides serving a novel and effective 3D-TM model, the MAX8B hydrogel could potentially function as an injectable trabecular meshwork implant.
Item Type: Article
Uncontrolled Keywords: Glaucoma; Injectable scaffold; IOP perfusion; Peptide hydrogel; Tissue engineering; Trabecular meshwork
Divisions: Sabancı University Nanotechnology Research and Application Center
Depositing User: Sibel Çetinel
Date Deposited: 31 Jul 2023 17:00
Last Modified: 31 Jul 2023 17:00

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