Boosting natural killer cell-mediated targeting of sarcoma through DNAM-1 and NKG2D

Sayitoglu, Ece Canan and Georgoudaki, Anna Maria and Chrobok, Michael and Özkazanç Ünsal, Didem and Josey, Benjamin J. and Arif, Muhammad and Kusser, Kim and Hartman, Michelle and Chinn, Tamara M. and Potens, Renee and Pamukcu, Cevriye and Krueger, Robin and Zhang, Cheng and Mardinoglu, Adil and Alici, Evren and Temple, Harry Thomas and Sutlu, Tolga and Duru, Adil Doganay (2020) Boosting natural killer cell-mediated targeting of sarcoma through DNAM-1 and NKG2D. Frontiers in Immunology, 11 . ISSN 1664-3224

Full text not available from this repository. (Request a copy)


Sarcomas are malignancies of mesenchymal origin that occur in bone and soft tissues. Many are chemo- and radiotherapy resistant, thus conventional treatments fail to increase overall survival. Natural Killer (NK) cells exert anti-tumor activity upon detection of a complex array of tumor ligands, but this has not been thoroughly explored in the context of sarcoma immunotherapy. In this study, we investigated the NK cell receptor/ligand immune profile of primary human sarcoma explants. Analysis of tumors from 32 sarcoma patients identified the proliferative marker PCNA and DNAM-1 ligands CD112 and/or CD155 as commonly expressed antigens that could be efficiently targeted by genetically modified (GM) NK cells. Despite the strong expression of CD112 and CD155 on sarcoma cells, characterization of freshly dissociated sarcomas revealed a general decrease in tumor-infiltrating NK cells compared to the periphery, suggesting a defect in the endogenous NK cell response. We also applied a functional screening approach to identify relevant NK cell receptor/ligand interactions that induce efficient anti-tumor responses using a panel NK-92 cell lines GM to over-express 12 different activating receptors. Using GM NK-92 cells against primary sarcoma explants (n = 12) revealed that DNAM-1 over-expression on NK-92 cells led to efficient degranulation against all tested explants (n = 12). Additionally, NKG2D over-expression showed enhanced responses against 10 out of 12 explants. These results show that DNAM-1+ or NKG2D+ GM NK-92 cells may be an efficient approach in targeting sarcomas. The degranulation capacity of GM NK-92 cell lines was also tested against various established tumor cell lines, including neuroblastoma, Schwannoma, melanoma, myeloma, leukemia, prostate, pancreatic, colon, and lung cancer. Enhanced degranulation of DNAM-1+ or NKG2D+ GM NK-92 cells was observed against the majority of tumor cell lines tested. In conclusion, DNAM-1 or NKG2D over-expression elicited a dynamic increase in NK cell degranulation against all sarcoma explants and cancer cell lines tested, including those that failed to induce a notable response in WT NK-92 cells. These results support the broad therapeutic potential of DNAM-1+ or NKG2D+ GM NK-92 cells and GM human NK cells for the treatment of sarcomas and other malignancies.
Item Type: Article
Uncontrolled Keywords: cancer immunology; cancer immunotherapy; DNAM-1 (CD226); natural killer (NK) cell; NK-92 cell line; NKG2D (Natural killer group 2 member D); sarcoma
Divisions: Faculty of Engineering and Natural Sciences
Depositing User: Didem Özkazanç Ünsal
Date Deposited: 29 Jul 2023 21:48
Last Modified: 29 Jul 2023 21:48

Actions (login required)

View Item
View Item