Application of tissue culture and transformation techniques in model species Brachypodium distachyon

Sogutmaz Ozdemir, Bahar and Budak, Hikmet (2018) Application of tissue culture and transformation techniques in model species Brachypodium distachyon. In: Sablok, Gaurav and Budak, Hikmet and Ralph, Peter J., (eds.) Brachypodium Genomics: Methods and Protocols. Methods in Molecular Biology, 1667. Humana, New York, NY, pp. 289-310. ISBN 978-1-4939-8439-8 (Print) 978-1-4939-7278-4 (Online)

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Brachypodium distachyon has recently emerged as a model plant species for the grass family (Poaceae) that includes major cereal crops and forage grasses. One of the important traits of a model species is its capacity to be transformed and ease of growing both in tissue culture and in greenhouse conditions. Hence, plant transformation technology is crucial for improvements in agricultural studies, both for the study of new genes and in the production of new transgenic plant species. In this chapter, we review an efficient tissue culture and two different transformation systems for Brachypodium using most commonly preferred gene transfer techniques in plant species, microprojectile bombardment method (biolistics) and Agrobacterium-mediated transformation. In plant transformation studies, frequently used explant materials are immature embryos due to their higher transformation efficiencies and regeneration capacity. However, mature embryos are available throughout the year in contrast to immature embryos. We explain a tissue culture protocol for Brachypodium using mature embryos with the selected inbred lines from our collection. Embryogenic calluses obtained from mature embryos are used to transform Brachypodium with both plant transformation techniques that are revised according to previously studied protocols applied in the grasses, such as applying vacuum infiltration, different wounding effects, modification in inoculation and cocultivation steps or optimization of bombardment parameters.
Item Type: Book Section / Chapter
Uncontrolled Keywords: Agrobacterium-mediated transformation; Brachypodium distachyon; Mature embryo-derived callus culture; Microprojectile bombardment (Biolistics); Plant tissue culture; Plant transformation
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng.
Faculty of Engineering and Natural Sciences
Depositing User: Hikmet Budak
Date Deposited: 14 Jun 2023 12:00
Last Modified: 14 Jun 2023 12:00

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