CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin

Rabiee, Navid and Akhavan, Omid and Fatahi, Yousef and Ghadiri, Amir Mohammad and Kiani, Mahsa and Makvandi, Pooyan and Rabiee, Mohammad and Nicknam, Mohammad Hossein and Saeb, Mohammad Reza and Varma, Rajender S. and Ashrafizadeh, Milad and Zare, Ehsan Nazarzadeh and Sharifi, Esmaeel and Lima, Eder C. (2022) CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin. Chemosphere, 306 . ISSN 0045-6535 (Print) 1879-1298 (Online)

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Overexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression of ssDNA and the related antigens in the population and brisk activity in gene-editing technologies in the treatment/detection may lead to the presence of pCRISPR in the blood. Therefore, the detection and evaluation of their trace concentrations are of critical importance. CaZnO-based nanoghosts (NGs) were synthesized with the assistance of a high-gravity technique at a 1,800 MHz field, capitalizing on the use of Rosmarinus officinalis leaf extract as the templating agent. A complete chemical, physical and biological investigation revealed that the synthesized NGs presented similar morphological features to the mesenchymal stem cells (MSCs), resulting in excellent biocompatibility, interaction with ssDNA- and/or pCRISPR-surface, through various chemical and physical mechanisms. This comprise the unprecedented synthesis of a fully inorganic nanostructure with behavior that is similar to MSCs. Furthermore, the endowed exceptional ability of inorganic NGs for detective sensing/folding of ssDNA and pCRISPR and recombinant SARS-CoV-2 spike antigen (RSCSA), along with in-situ hydrogen peroxide detection on the HEK-293 and HeLa cell lines, was discerned. On average, they displayed a high drug loading capacity of 55%, and the acceptable internalizations inside the HT-29 cell lines affirmed the anticipated MSCs-like behavior of these inorganic-NGs.
Item Type: Article
Uncontrolled Keywords: Gene delivery; In-situ biosensor; Nanoghosts; pCRISPR; SARS-CoV-2; ssDNA
Divisions: Faculty of Engineering and Natural Sciences
Depositing User: Milad Ashrafizadeh
Date Deposited: 20 Aug 2022 15:02
Last Modified: 20 Aug 2022 15:02

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