Detection of dopamine receptors using nanoscale dendrimer for potential application in targeted delivery and whole-body imaging: synthesis and in vivo organ distribution

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Ramezani Farani, Marzieh and Aminzadeh Jahromi, Negin and Ali, Vahid and Ebrahimpour, Anita and Salehian, Elnaz and Shafiee Ardestani, Mehdi and Seyedhamzeh, Mohammad and Ahmadi, Sepideh and Sharifi, Esmaeel and Ashrafizadeh, Milad and Rabiee, Navid and Makvandi, Pooyan (2022) Detection of dopamine receptors using nanoscale dendrimer for potential application in targeted delivery and whole-body imaging: synthesis and in vivo organ distribution. ACS Applied Bio Materials, 5 (4). pp. 1744-1755. ISSN 2576-6422

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Abstract

Dopamine is one of the most important neurotransmitters released by neurons in the central nervous system, and a variety of neurological illnesses and mental disorders are associated with impairments in the secretion and functionality of dopamine. Dopamine, depending on the type of receptors, can act as a stimulant or an inhibitor. In this study, dendrimer-conjugated dopamine was utilized as a chelating agent for Technetium-99m to investigate the organ distribution of this compound in vivo using the single-photon emission computed tomography (SPECT) technique. For this purpose, dendrimers were synthesized using polyethylene glycol diacid and citric acid precursors, and dopamine was conjugated to the dendrimer using EDC/NHS cross-linker. The results showed no sign of toxicity of the dopamine-functionalized dendrimers on HEK-293 cell lines. The optimization of labeling conditions was conducted using the experimental design method (i.e., conjugate value, pH, and the amount of reducing agent), and then labeling efficiency was evaluated by thin-layer chromatography (TLC). Finally, the study of organ distribution in normal mice using SPECT imaging and comparing it with gene expression in different organs revealed that dopamine D1 receptors exhibited the highest accumulation in the liver and that the drug retained its specificity.
Item Type: Article
Uncontrolled Keywords: central nervous system; dendrimer; dopamine; polyethylene glycol; SPECT imaging; Technetium-99m
Divisions: Faculty of Engineering and Natural Sciences
Depositing User: Milad Ashrafizadeh
Date Deposited: 22 Aug 2022 20:25
Last Modified: 22 Aug 2022 20:25
URI: https://research.sabanciuniv.edu/id/eprint/44132

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