Bioactive hybrid metal-organic framework (MOF)-based nanosensors for optical detection of recombinant SARS-CoV-2 spike antigen

Rabiee, Navid and Fatahi, Yousef and Ahmadi, Sepideh and Abbariki, Nikzad and Ojaghi, Amirhossein and Rabiee, Mohammad and Radmanesh, Fatemeh and Dinarvand, Rassoul and Bagherzadeh, Mojtaba and Mostafavi, Ebrahim and Ashrafizadeh, Milad and Makvandi, Pooyan and Lima, Eder C. and Saeb, Mohammad Reza (2022) Bioactive hybrid metal-organic framework (MOF)-based nanosensors for optical detection of recombinant SARS-CoV-2 spike antigen. Science of the Total Environment, 825 . ISSN 0048-9697 (Print) 1879-1026 (Online)

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Fast, efficient, and accurate detection of SARS-CoV-2 spike antigen is pivotal to control the spread and reduce the mortality of COVID-19. Nevertheless, the sensitivity of available nanobiosensors to detect recombinant SARS-CoV-2 spike antigen seems insufficient. As a proof-of-concept, MOF-5/CoNi2S4 is developed as a low-cost, safe, and bioactive hybrid nanostructure via the one-pot high-gravity protocol. Then, the porphyrin, H2TMP, was attached to the surface of the synthesized nanomaterial to increase the porosity for efficient detection of recombinant SARS-CoV-2 spike antigen. AFM results approved roughness in different ranges, including 0.54 to 0.74 μm and 0.78 to ≈0.80 μm, showing good physical interactions with the recombinant SARS-CoV-2 spike antigen. MTT assay was performed and compared to the conventional synthesis methods, including hydrothermal, solvothermal, and microwave-assisted methods. The synthesized nanodevices demonstrated above 88% relative cell viability after 24 h and even 48 h of treatment. Besides, the ability of the synthesized nanomaterials to detect the recombinant SARS-CoV-2 spike antigen was investigated, with a detection limit of 5 nM. The in-situ synthesized nanoplatforms exhibited low cytotoxicity, high biocompatibility, and appropriate tunability. The fabricated nanosystems seem promising for future surveys as potential platforms to be integrated into biosensors.
Item Type: Article
Uncontrolled Keywords: Biosensor; COVID-19; High-gravity technique; MOF-5; Recombinant SARS-CoV-2 spike antigen
Divisions: Faculty of Engineering and Natural Sciences
Sabancı University Nanotechnology Research and Application Center
Depositing User: Milad Ashrafizadeh
Date Deposited: 25 Aug 2022 09:06
Last Modified: 25 Aug 2022 09:06

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