Bisulfite-initiated crosslinking of gelatin methacryloyl hydrogels for embedded 3D bioprinting

Bilici, Çiğdem and Tatar, Asena Gülenay and Şentürk, Efsun and Dikyol, Caner and Koç, Bahattin (2022) Bisulfite-initiated crosslinking of gelatin methacryloyl hydrogels for embedded 3D bioprinting. Biofabrication, 14 (2). ISSN 1758-5082 (Print) 1758-5090 (Online)

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Recent studies on three-dimensional (3D) bioprinting of cell-laden gelatin methacryloyl (GelMA) hydrogels have provided promising outcomes for tissue engineering applications. However, the reliance on the use of photo-induced gelation processes for the bioprinting of GelMA and the lack of an alternative crosslinking process remain major challenges for the fabrication of cell-laden structures. Here, we present a novel crosslinking approach to form cell-laden GelMA hydrogel constructs through 3D embedded bioprinting without using any external irradiation that could drastically affect cell viability and functionality. This approach consists of a one-step type of crosslinking via bisulfite-initiated radical polymerization, which is combined with embedded bioprinting technology to improve the structural complexity of printed structures. By this means, complex-shaped hydrogel bio-structures with cell viability higher than 90% were successfully printed within a support bath including sodium bisulfite. This study offers an important alternative to other photo-induced gelation processes to improve the bio-fabrication of GelMA hydrogel with high cell viability.
Item Type: Article
Uncontrolled Keywords: bisulfite; crosslinking; embedded bioprinting; GelMA; hydrogel; support bath
Divisions: Faculty of Engineering and Natural Sciences
Sabancı University Nanotechnology Research and Application Center
Integrated Manufacturing Technologies Research and Application Center
Depositing User: Bahattin Koç
Date Deposited: 25 Aug 2022 12:57
Last Modified: 25 Aug 2022 12:57

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