A low-cost, portable, and practical LAMP device for point-of-diagnosis in the field

Vural Kaymaz, Sümeyra and Ergenç, Ali F. and Aytekin, Ali and Lucas, Stuart J. and Elitaş, Meltem (2022) A low-cost, portable, and practical LAMP device for point-of-diagnosis in the field. Biotechnology and Bioengineering, 119 (3). pp. 994-1003. ISSN 0006-3592 (Print) 1097-0290 (Online)

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Transition of rapid, ready-to-use, and low-cost nucleic acid-based detection technologies from laboratories to points of sample collection has drastically accelerated. However, most of these approaches are still incapable of diagnosis starting from sampling through nucleic acid isolation and detection in the field. Here we developed a simple, portable, low-cost, colorimetric, and remotely controllable platform for reliable, high-throughput, and rapid diagnosis using loop-mediated isothermal amplification (LAMP) assays. It consists of a thermally isolated cup, low-cost electronic components, a polydimethylsiloxane sample well, and a fast prototyped case that covers electronic components. The steady-state temperature error of the system is <1%. We performed LAMP, Colony-LAMP, and Colony polymerase chain reactions (PCRs) using the yaiO2 primer set for Escherichia coli and Pseudomonas aeruginosa samples at 65°C and 30 min. We detected the end-point colorimetric readouts by the naked eye under day light. We confirmed the specificity and sensitivity of our approach using pure genomic DNA and crude bacterial colonies. We benchmarked our Colony-LAMP detection against Colony PCR. The number of samples tested can easily be modified for higher throughput in our system. We strongly believe that our platform can greatly contribute rapid and reliable diagnosis in versatile operational environments.
Item Type: Article
Uncontrolled Keywords: colony-PCR; field; high-throughput; LAMP; remote control
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Mechatronics
Faculty of Engineering and Natural Sciences
Sabancı University Nanotechnology Research and Application Center
Depositing User: Meltem Elitaş
Date Deposited: 26 Aug 2022 17:18
Last Modified: 26 Aug 2022 17:18
URI: https://research.sabanciuniv.edu/id/eprint/43931

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