A bioengineering method for modeling alveolar Rhabdomyosarcoma and assessing chemotherapy responses

Stefanek, Evan and Samiei, Ehsan and Kavoosi, Mahboubeh and Esmaeillou, Mohammad and Roustai Geraylow, Kiarash and Emami, Arya and Ashrafizadeh, Milad and Perrin, David and Gordon, Joseph W. and Akbari, Mohsen and Ghavami, Saeid (2021) A bioengineering method for modeling alveolar Rhabdomyosarcoma and assessing chemotherapy responses. MethodsX, 8 . ISSN 2215-0161

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Rhabdomyosarcoma (RMS) is the most common pediatric soft-tissue malignant tumor. Treatment of RMS usually includes primary tumor resection along with systemic chemotherapy. Two-dimensional (2D) cell culture systems and animal models have been extensively used for investigating the potential efficacy of new RMS treatments. However, RMS cells behave differently in 2D culture than in vivo, which has recently inspired the adoption of three-dimensional (3D) culture environments. In the current paper, we will describe the detailed methodology we have developed for fabricating a 3D engineered model to study alveolar RMS (ARMS) in vitro. This model consists of a thermally cross-linked collagen disk laden with RMS cells that mimics the structural and bio-chemical aspects of the tumor extracellular matrix (ECM). This process is highly reproducible and produces a 3D engineered model that can be used to analyze the cytotoxicity and autophagy induction of drugs on ARMS cells. The most improtant bullet points are as following: • We fabricated 3D model of ARMS. • The current ARMS 3D model can be used for screening of chemotherapy drugs. • We developed methods to detect apoptosis and autophagy in ARMS 3D model to detect the mechansims of chemotherapy agents.
Item Type: Article
Uncontrolled Keywords: Alveolar Rhabdomyosarcoma 3D model; Apoptosis; Autophagy; Biofabrication; Cell death; Rhabdomyosarcoma
Divisions: Faculty of Engineering and Natural Sciences
Depositing User: Milad Ashrafizadeh
Date Deposited: 28 Aug 2022 18:10
Last Modified: 28 Aug 2022 18:10
URI: https://research.sabanciuniv.edu/id/eprint/43783

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