Curcumin efficacy in a serum/glucose deprivation-induced neuronal PC12 injury model

Farkhondeh, Tahereh and Ashrafizadeh, Milad and Azimi-Nezhad, Mohsen and Samini, Fariborz and Aschner, Michael and Samarghandian, Saeed (2021) Curcumin efficacy in a serum/glucose deprivation-induced neuronal PC12 injury model. Current Molecular Pharmacology, 14 (6). pp. 1146-1155. ISSN 1874-4672 (Print) 1874-4702 (Online)

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Background: Glucose/serum deprivation (GSD), has been used for understanding molecular mechanisms of neuronal damage during ischemia. It has been suggested that curcumin may improve neurodegenerative diseases. Aim: In this study, the protective effects of curcumin and its underlying mechanisms were investi-gated in PC12 cells upon GSD-induced stress. Methods: PC12 cells were cultured in DMEM overnight and then incubated in GSD condition for either 6 or 12h. GSD-treated cells were pretreated with various concentrations of curcumin (10, 20, and 40 μM) for 5h. The cell viability, apoptosis, reactive oxygen species (ROS) level, oxidative stress, expression of apoptosis-related genes, and IL-6 were determined. Results: Curcumin increased cell viability and caused an anti-apoptotic effect in PC12 cells exposed for 12h to GSD . Curcumin also increased antioxidant enzyme expression, suppressed lipid peroxidation, and decreased interleukin-6 secretion in PC12 cells subjected to GSD. In addition, pretreatment with curcumin down-regulated pro-apoptotic (Bax), and up-regulated antiapoptotic (Bcl2) mediators. Conclusion: Curcumin mitigates many of the adverse effects of ischemia, and therefore, should be considered as an adjunct therapy in ischemic patients.
Item Type: Article
Uncontrolled Keywords: Apoptosis; Curcumin; Cytotoxicity; Inflammation; Oxidative stress; PC12 cell; Serum/glucose deprivation
Divisions: Faculty of Engineering and Natural Sciences
Depositing User: Milad Ashrafizadeh
Date Deposited: 31 Aug 2022 13:33
Last Modified: 31 Aug 2022 13:33

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