Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles

Damavandi, Fereshte and Wang, Weiwei and Shen, Wei Zheng and Çetinel, Sibel and Jordan, Tracy and Jovel, Juan and Montemagno, Carlo and Wong, Gane Ka Shu (2021) Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles. Scientific Reports, 11 (1). ISSN 2045-2322

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Detection of low abundance target DNA/RNA for clinical or research purposes is challenging because the target sequences can be hidden under a large background of human genomic or non-human metagenomic sequences. We describe a probe-based capture method to enrich for target sequences with DNA-clicked iron oxide nanoparticles. Our method was tested against commercial capture assays using streptavidin beads, on a set of probes derived from a common genotype of the hepatitis C virus. We showed that our method is more specific and sensitive, most likely due to the combination of an inert silica coating and a high density of DNA probes clicked to the nanoparticles. This facilitates target capture below the limits of detection for TaqMan qPCR, and we believe that this method has the potential to transform management of infectious diseases.
Item Type: Article
Divisions: Sabancı University Nanotechnology Research and Application Center
Depositing User: Sibel Çetinel
Date Deposited: 01 Sep 2022 20:52
Last Modified: 01 Sep 2022 20:52
URI: https://research.sabanciuniv.edu/id/eprint/43522

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