Antibacterial hybrid coatings from halloysite-immobilized lysostaphin and waterborne polyurethanes

Alkan Taş, Buket and Durmuş Sayar, Ayşe and Duman, Z. Efsun and Seviniş, Emine Billur and Unlu, Aise and Binay, Barış and Ünal, Serkan and Ünal, Hayriye (2021) Antibacterial hybrid coatings from halloysite-immobilized lysostaphin and waterborne polyurethanes. Progress in Organic Coatings, 156 . ISSN 0300-9440 (Print) 1873-331X (Online)

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Lysostaphin enzyme is an effective antibacterial agent proven to be promising for the prevention of healthcare-associated infections related to methicillin-resistant Staphylococcus aureus (MRSA). Here, safe, and non-toxic antibacterial hybrid coatings consisting of lysostaphin and waterborne polyurethane (PU) are presented. Lysostaphin was covalently immobilized onto polydopamine functionalized halloysite nanotubes (HNTs) to obtain stable HNT-lysostaphin nanohybrid structures with high effective lysostaphin concentrations, which were then incorporated into PU coatings by a facile spray coating process. Resulting PU/HNT-lysostaphin hybrid coatings presented strong antibacterial activity against S. aureus with a >99 % killing efficiency. The incorporation of lysostaphin into the polymer matrix via HNTs as a carrier enhanced the stability of the enzyme resulting in non-leaching coatings that presented high operational stability over multiple bacterial incubation cycles and high storage stability without any significant loss of enzymatic activity. Furthermore, PU/HNT-lysostaphin coatings demonstrated significant antibiofilm properties and reduced the formation of S. aureus biofilms by 70 % relative to neat PU coatings. The lysostaphin based antibacterial hybrid coatings developed in this study, which can be easily applied to surfaces in healthcare facilities and medical devices offer an effective approach for the prevention of S. aureus associated nosocomial infections.
Item Type: Article
Uncontrolled Keywords: Antibacterial coating; Halloysite nanotubes; Lysostaphin; Methicillin-resistant Staphylococcus aureus (MRSA)); Polydopamine
Divisions: Faculty of Engineering and Natural Sciences > Basic Sciences > Chemistry
Faculty of Engineering and Natural Sciences > Academic programs > Materials Science & Eng.
Faculty of Engineering and Natural Sciences
Sabancı University Nanotechnology Research and Application Center
Integrated Manufacturing Technologies Research and Application Center
Depositing User: Serkan Ünal
Date Deposited: 17 Aug 2022 15:01
Last Modified: 17 Aug 2022 15:01

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