An interaction matrix of BTB domain-containing transcription factors

Noğay, Liyne (2019) An interaction matrix of BTB domain-containing transcription factors. [Thesis]

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The Bric-a-brac, Tramtrack, and Broad (BTB) domain is a protein-protein interaction unit found in eukaryotes. It forms a distinct multimeric structure with a large interaction surface. The exposed residues of each monomer are highly variable and can allow dimerization, oligomerization, and interactions with several other proteins such as the corepressors nuclear receptor corepressor (NCOR) and silencing mediator of retinoic acid and thyroid hormone receptor (SMRT). BTB-containing transcription factors are diverse and control various physiological processes ranging from immune system development to cell cycle regulation. To understand the structural basis of these functions, we assessed the interaction networks of these proteins. In this study, we developed specific and systematic assays to screen the interactions between various BTB domain-containing transcription factors and their interaction partners in vitro and in vivo, using surface plasmon resonance (SPR) and fluorescent two-hybrid (F2H) assays. We constructed a homo- and hetero-dimerization matrix of several BTB domains of interest.
Item Type: Thesis
Uncontrolled Keywords: BTB domain. -- Transcription factors. -- NCOR/SMRT corepressors. -- Surface plasmon resonance. -- Fluorescent two-hybrid assay. -- BTB bölgesi. -- Transkripsiyon faktörleri. -- NCOR/SMRT eşbaskılayıcıları. -- Yüzey plasmon rezonans. -- Flörosan ikili hibrit tekniği.
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng.
Faculty of Engineering and Natural Sciences
Depositing User: IC-Cataloging
Date Deposited: 25 Feb 2020 14:24
Last Modified: 26 Apr 2022 10:32

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