Alternating magnetic field plate for enhanced magnetofection of iron oxide nanoparticle conjugated nucleic acids

Warning The system is temporarily closed to updates for reporting purpose.

Yapıcı, Murat Kaya and Al Nabulsi, Ahmad and Rizk, Nahla and Mokhtar Boularaoui, Selwa and Christoforou, Nicolas and Lee, Sung Mun (2019) Alternating magnetic field plate for enhanced magnetofection of iron oxide nanoparticle conjugated nucleic acids. Journal of Magnetism and Magnetic Materials, 469 . pp. 598-605. ISSN 0304-8853 (Print) 1873-4766 (Online)

This is the latest version of this item.

Full text not available from this repository. (Request a copy)

Abstract

Magnet-assisted transfection or magnetofection refers to the delivery of nucleic acids to target cells with the help of conjugated superparamagnetic iron oxide nanoparticles (SPIONs) and external magnetic fields generated by permanent magnet plates. The external magnetic field, among other parameters, directly affects the transfection efficiency. However, standard permanent magnet plates generate static magnetic fields which are not as effective compared to time-varying, dynamic fields. In this work, we show a new and novel "AC magnet plate" compatible with standard 96-well cell culture plates, which can be easily adapted for benchtop use in a typical lab setting. We provide full design details, modelling, fabrication, measurement and testing on human embryonic kidney cells (HEK 293) to show transfection improvement. We perform magnetofection under different field conditions and show that with increasing AC content, efficiency of transfection is improved.
Item Type: Article
Uncontrolled Keywords: Magnetofection; Alternating field; Dynamic field; Oscillating field; Gene delivery; Nucleic acid; Magnetic transfection; Magnet plate; Electromagnet; AC; Superparamagnetic iron oxide nanoparticle; SPION; HEK 293
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Electronics
Faculty of Engineering and Natural Sciences
Depositing User: Murat Kaya Yapıcı
Date Deposited: 21 Aug 2019 22:49
Last Modified: 05 Jun 2023 15:04
URI: https://research.sabanciuniv.edu/id/eprint/38194

Available Versions of this Item

Actions (login required)

View Item
View Item