Exploiting stokes and anti-stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applications

Yüce, Meral and Kurt, Hasan and Hussain, Babar and Ow-Yang, Cleva W. and Budak, Hikmet (2018) Exploiting stokes and anti-stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applications. Chemistry Select, 3 (21). pp. 5814-5823. ISSN 2365-6549

Full text not available from this repository. (Request a copy)

Abstract

Multiplex biosensing of four types of bacterial food pathogens in one pot is described. Stokes and anti-Stokes type photoluminescence (PL) of two quantum dots (QD) and two upconverting nanoparticles (UCNP) were utilised for realisation of the multiplex detection. The biosensing system was composed of aptamer-functionalized QD and UCNP nanoprobes that were conjugated with partially complementary DNA-modified magnetic beads for separation. PL signals of the conjugates were collected before and after incubation with target pathogens in one pot through the sequential excitations at 335nm for QD probes and 980nm for UCNP probes. The limit of detection values achieved were 28, 15, 12 and 25 cfu center dot mL(-1) for Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa, respectively. Efforts for extending the multiplex detection up to five pathogens were also presented even though the PL signal cross-talk of a third QD nanoprobe hindered the detection. This work empowers co-deployment of QDs and UCNPs and paves the way for future studies in multiplex sensing, labelling, and bioimaging fields.
Item Type: Article
Uncontrolled Keywords: Aptamers; Biosensor; Foodborne Pathogens; Quantum Dots; Upconverting Nanoparticles
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Q Science > QC Physics
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Materials Science & Eng.
Sabancı University Nanotechnology Research and Application Center
Faculty of Engineering and Natural Sciences
Depositing User: Cleva W. Ow-Yang
Date Deposited: 12 Aug 2018 23:33
Last Modified: 27 May 2023 21:57
URI: https://research.sabanciuniv.edu/id/eprint/34967

Actions (login required)

View Item
View Item