FbpA iron storage and release are governed by periplasmic microenvironments

Şensoy, Özge and Atılgan, Ali Rana and Atılgan, Canan (2017) FbpA iron storage and release are governed by periplasmic microenvironments. Physical Chemistry Chemical Physics, 19 (8). pp. 6064-6075. ISSN 1463-9076 (Print) 1463-9084 (Online)

This is the latest version of this item.

[thumbnail of submitted manuscript] PDF (submitted manuscript)
Restricted to Repository staff only

Download (1MB) | Request a copy


Ferric binding protein (FbpA) is part of an elaborate iron piracy mechanism evolved in Gram-negative bacteria, shuttling iron in the periplasmic space, from the outer to the cytoplasmic membrane side. We address how the dissociation process of iron is facilitated, since the binding constant of iron is on the order of 1018 M 1 at 6.5 pH and 200 mM ionic strength (IS). We monitor the conformational preferences of FbpA by extensive molecular dynamics (MD) simulations under conditions where IS, charge states of iron coordinating tyrosines and pH are varied, as well as when a mutation is introduced at an allosteric site. Steered MD is utilized to predict the binding affinity of iron. After triggering lobe opening by changing the charge states of tyrosines, the conformations adopted and the iron binding affinity still depend on pH, IS and allosteric interactions. To relate the observed conformational changes to the environmental conditions that might be encountered in the periplasmic space, we offer a plausible model that couples electrostatic potential distribution to the mechanical motions invoked. Although low pH/IS and allosteric perturbations decrease the affinity of iron, it remains high for spontaneous dissociation. However, the conformational changes modulated by the environmental conditions expose iron for chelation. Our study provides a quantitative dimension and molecular details to interpret the contribution of possible environmental conditions present in the periplasmic space to iron dissociation from FbpA, opening up the opportunity of modulating function via allosteric mutations or altering environmental conditions, thus offering a new route to developing strategies towards antibiotic resistance by targeting nutritional requirements.
Item Type: Article
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Materials Science & Eng.
Faculty of Engineering and Natural Sciences
Depositing User: Canan Atılgan
Date Deposited: 20 Apr 2017 15:33
Last Modified: 26 Apr 2022 09:42
URI: https://research.sabanciuniv.edu/id/eprint/31158

Available Versions of this Item

Actions (login required)

View Item
View Item