Evaluation of cytotoxic, oxidative stress and genotoxic responses of hydroxyapatite nanoparticles on human blood cells

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Turkez, Hasan and Yousef, Mokhtar I. and Sönmez, Erdal and Togar, Başak and Bakan, Feray and Sozio, Piera and Di Stefano, Antonio (2013) Evaluation of cytotoxic, oxidative stress and genotoxic responses of hydroxyapatite nanoparticles on human blood cells. Journal of Applied Toxicology . ISSN 0260-437X (Print) 1099-1263 (Online) Published Online First http://dx.doi.org/10.1002/jat.2958

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Abstract

The present study was designed to investigate genotoxic and cytotoxic effects and oxidative damage of increasing concentrations of nano-hydroxyapatite (5, 10, 20, 50, 75, 100, 150, 300, 500 and 1000 ppm) in primary human blood cell cultures. Cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] assay and lactate dehydrogenase release, while total antioxidant capacity and total oxidative stress levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by sister chromatid exchange, micronuclei, chromosome aberration assays and 8-oxo-2-deoxyguanosine level as indicators of genotoxicity. The results of [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] and lactate dehydrogenase assays showed that the higher concentrations (150, 300, 500 and 1000 ppm) of hydroxyapatite nanoparticles (HAP NPs) decreased cell viability. HAP NPs led to increases of total oxidative stress (300, 500 and 1000 ppm) levels and decreased total antioxidant capacity (150, 300, 500 and 1000 ppm) levels in cultured human blood cells. On the basis of increasing concentrations, HAP NPs caused significant increases of sister chromatid exchange, micronuclei, chromosome aberration rates and 8-oxo-2-deoxyguanosine levels as compared to untreated culture. In conclusion, the obtained in vitro results showed that HAP NPs had dose-dependent effects on inducing oxidative damage, genotoxicity and cytotoxicity in human blood cells.
Item Type: Article
Uncontrolled Keywords: Hydroxyapatite; Genotoxicity; Cytotoxicity; Lymphocyte; Oxidative stress
Divisions: Sabancı University Nanotechnology Research and Application Center
Depositing User: Feray Bakan
Date Deposited: 17 Jan 2014 21:00
Last Modified: 26 Apr 2022 09:09
URI: https://research.sabanciuniv.edu/id/eprint/23087

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