A simple guide for generating BAC transgenic animals for retinal research
Ağca, Cavit and Grimm, Christian (2020) A simple guide for generating BAC transgenic animals for retinal research. In: Mao, Chai-An, (ed.) Retinal Development: Methods and Protocols. Methods in Molecular Biology, 2092. Springer, US, pp. 109-122. ISBN 978-1-0716-0174-7 (Print) 978-1-0716-0175-4 (Online)
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Official URL: http://dx.doi.org/10.1007/978-1-0716-0175-4_9
Bacterial artificial chromosomes (BACs) are genomic tools that can carry several hundred kilobases of exogenous genomic material. This allows to incorporate sufficiently large DNA stretches to include most if not all upstream and downstream cis-regulatory elements of a gene in order to mimic and analyze its endogenous regulation of expression using a reporter protein in vivo. Here, we illustrate the generation of a BAC:LIF-EGFP transgenic mouse line to describe a simplified version of BAC transgenesis using galK-based recombineering.
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