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CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement

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Hussain, Babar and Lucas, Stuart J. and Budak, Hikmet (2018) CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement. Briefings in Functional Genomics, 17 (5). pp. 319-328. ISSN 2041-2649 (Print) 2041-2657 (Online)

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Official URL: http://dx.doi.org/10.1093/bfgp/ely016

Abstract

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system uses single-guide RNAs for genome editing, making it a simple, robust, powerful tool for targeted gene mutagenesis, knockout and knock-in/replacement, as well as transcriptional regulation. Here, we review the working principles, components and potential modifications of CRISPR/Cas9 for efficient single and multiplex gene editing in plants. We also describe recent work that has used CRISPR/Cas9 to improve economically important traits in crop plants. Although the apparent ease of CRISPR/Cas9-mediated editing may make it appear as though scientists are merely playing with plant genomes, the combined power of CRISPR/Cas9 has enabled vital research to be completed in the battle toward optimization and adaptation of crop species, permitting crucial advances to be achieved in crop improvement.

Item Type:Article
Uncontrolled Keywords:CRISPR/Cas9; crop improvement; food nutrition; climate-resilient crops; plant defense response
Subjects:Q Science > QH Natural history > QH506 Molecular biology
ID Code:38319
Deposited By:Stuart J. Lucas
Deposited On:07 Aug 2019 19:53
Last Modified:07 Aug 2019 19:53

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