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Alternating magnetic field plate for enhanced magnetofection of iron oxide nanoparticle conjugated nucleic acids

Yapıcı, Murat Kaya and Al Nabulsi, Ahmad and Rizk, Nahla and Mokhtar Boularaoui, Selwa and Christoforou, Nicolas and Lee, Sung Mun (2019) Alternating magnetic field plate for enhanced magnetofection of iron oxide nanoparticle conjugated nucleic acids. Journal of Magnetism and Magnetic Materials, 469 . pp. 598-605. ISSN 0304-8853 (Print) 1873-4766 (Online)

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Official URL: http://dx.doi.org/10.1016/j.jmmm.2018.09.038

Abstract

Magnet-assisted transfection or magnetofection refers to the delivery of nucleic acids to target cells with the help of conjugated superparamagnetic iron oxide nanoparticles (SPIONs) and external magnetic fields generated by permanent magnet plates. The external magnetic field, among other parameters, directly affects the transfection efficiency. However, standard permanent magnet plates generate static magnetic fields which are not as effective compared to time-varying, dynamic fields. In this work, we show a new and novel "AC magnet plate" compatible with standard 96-well cell culture plates, which can be easily adapted for benchtop use in a typical lab setting. We provide full design details, modelling, fabrication, measurement and testing on human embryonic kidney cells (HEK 293) to show transfection improvement. We perform magnetofection under different field conditions and show that with increasing AC content, efficiency of transfection is improved.

Item Type:Article
Uncontrolled Keywords:Magnetofection; Alternating field; Dynamic field; Oscillating field; Gene delivery; Nucleic acid; Magnetic transfection; Magnet plate; Electromagnet; AC; Superparamagnetic iron oxide nanoparticle; SPION; HEK 293
Subjects:UNSPECIFIED
ID Code:38194
Deposited By:Murat Kaya Yapıcı
Deposited On:21 Aug 2019 22:49
Last Modified:21 Aug 2019 22:49

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