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Enhancing enzymatic properties of endoglucanase I enzyme from trichoderma reesei via swapping from cellobiohydrolase I enzyme

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Yenenler, Aslı and Kurt, Hasan and Sezerman, Osman Uğur (2019) Enhancing enzymatic properties of endoglucanase I enzyme from trichoderma reesei via swapping from cellobiohydrolase I enzyme. Catalysts, 9 (2). ISSN 2073-4344

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Official URL: http://dx.doi.org/10.3390/catal9020130

Abstract

Utilizing plant-based materials as a biofuel source is an increasingly popular attempt to redesign the global energy cycle. This endeavour underlines the potential of cellulase enzymes for green energy production and requires the structural and functional engineering of natural enzymes to enhance their utilization. In this work, we aimed to engineer enzymatic and functional properties of Endoglucanase I (EGI) by swapping the Ala43-Gly83 region of Cellobiohydrolase I (CBHI) from Trichoderma reesei. Herein, we report the enhanced enzymatic activity and improved thermal stability of the engineered enzyme, called EGI_swapped, compared to EGI. The difference in the enzymatic activity profile of EGI_swapped and the EGI enzymes became more pronounced upon increasing metal-ion concentrations in the reaction media. Notably, the engineered enzyme retained a considerable level of enzymatic activity after thermal incubation for 90 min at 70 degrees C while EGI completely lost its enzymatic activity. Circular Dichroism spectroscopy studies revealed distinctive conformational and thermal susceptibility differences between EGI_swapped and EGI enzymes, confirming the improved structural integrity of the swapped enzyme. This study highlights the importance of swapping the metal-ion coordination region in the engineering of EGI enzyme for enhanced structural and thermal stability.

Item Type:Article
Uncontrolled Keywords:Endoglucanese I; Cellbiohydrolase I; Trichoderma reesei; Swapping; Protein Engineering; divalent metal ion
Subjects:UNSPECIFIED
ID Code:37030
Deposited By:Aslı Yenenler
Deposited On:21 May 2019 11:50
Last Modified:22 May 2019 14:14

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