Determination of ultrastructural properties of human carotid atherosclerotic plaques by scanning acoustic microscopy, micro-computer tomography, scanning electron microscopy and energy dispersive x-ray spectroscopy

Bilen, Bukem and Sener, Leyla Türker and Albeniz, Işıl and Sezen, Meltem and Ünlü, Mehmet Burçin and Uğurlucan, Murat (2019) Determination of ultrastructural properties of human carotid atherosclerotic plaques by scanning acoustic microscopy, micro-computer tomography, scanning electron microscopy and energy dispersive x-ray spectroscopy. Scientific Reports, 9 (1). ISSN 2045-2322

Full text not available from this repository. (Request a copy)

Abstract

Microcalcification is the precursor of vulnerability of plaques in humans. Visualization of such small structures in vivo with high spatial resolution is an unsolved issue. The goal of this study is to evaluate the potential of scanning acoustic microscopy (SAM) in the determination of atherosclerotic plaques with calcifications by validating this technique with micro-computer tomography (micro-CT), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The fibrocalcific plaques were obtained from 12 different patients and initially examined with micro-CT. The images exhibited calcifications within these plaques. For imaging with SAM, approximately 5 mu m thick slices were prepared. Sound speed values within calcified regions were measured to be greater than the ones in collagen-rich regions. These fibrocalcific plaques were also examined with SEM and EDS revealing collagen and calcium deposition within these samples. The consistency of the results obtained by all of the modalities involved in our study is an indication of the potential of SAM as a clinical tool for the diagnosis of vulnerable plaques.
Item Type: Article
Divisions: Sabancı University Nanotechnology Research and Application Center
Faculty of Engineering and Natural Sciences
Depositing User: Meltem Sezen
Date Deposited: 12 Feb 2019 15:49
Last Modified: 08 Jun 2023 14:51
URI: https://research.sabanciuniv.edu/id/eprint/36836

Actions (login required)

View Item
View Item