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Resveratrol protects against 4-HNE induced oxidative stress and apoptosis in Swiss 3T3 fibroblasts

Kütük, Özgür and Adli, Mazhar and Poli, Giuseppe and Başağa, Hüveyda (2004) Resveratrol protects against 4-HNE induced oxidative stress and apoptosis in Swiss 3T3 fibroblasts. Biofactors, 20 (1). pp. 1-10. ISSN 0951-6433

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Official URL: http://dx.doi.org/10.1002/biof.5520200101

Abstract

Here we report on the marked protective effect of resveratrol on 4-hydroxynonenal (4-HNE) induced oxidative stress and apoptotic death in Swiss 3T3 fibroblasts. 4-HNE, one of the major aldehydic products of the peroxidation of membrane w-6 polyunsaturated fatty acids, has been suggested to contribute to oxidant stress mediated cell injury. Indeed, in vitro treatment of 3T3 fibroblasts with 4-HNE induced a condition of oxidative stress as monitored by the oxidation of dichlorofluorescein diacetate; this reaction was prevented when cells were pretreated with resveratrol. Further, 4-HNE-treated fibroblasts eventually underwent apoptotic death as determined by differential staining and internucleosomal DNA fragmentation. Resveratrol pretreatment also prevented 4-HNE induced DNA fragmentation and apoptosis. These observations are consistent with a potential role of lipid peroxidation-derived products in programmed cell death and demonstrate that resveratrol can counteract this effect by quenching cell oxidative stress.

Item Type:Article
Uncontrolled Keywords:4-HNE; lipid peroxidation; cell death; resveratrol; DNA fragmentation
Subjects:Q Science > Q Science (General)
ID Code:428
Deposited By:Hüveyda Başağa
Deposited On:16 Feb 2007 02:00
Last Modified:25 May 2011 14:05

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