Computational model-informed design and bioprinting of cell-patterned constructs for bone tissue engineering

Carlier, Aurelli and Akdeniz Skvortsov, Gözde and Hafezi, Forough and Ferraris, Eleonora and Patterson, Jennifer and Koç, Bahattin and Oosterwyck, Hans Van (2016) Computational model-informed design and bioprinting of cell-patterned constructs for bone tissue engineering. Biofabrication, 8 (2). ISSN 1758-5082 (Print) 1758-5090 (Online)

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Official URL: http://dx.doi.org/10.1088/1758-5090/8/2/025009


Three-dimensional (3D) bioprinting is a rapidly advancing tissue engineering technology that holds great promise for the regeneration of several tissues, including bone. However, to generate a successful 3D bone tissue engineering construct, additional complexities should be taken into account such as nutrient and oxygen delivery, which is often insufficient after implantation in large bone defects. We propose that a well-designed tissue engineering construct, that is, an implant with a specific spatial pattern of cells in a matrix, will improve the healing outcome. By using a computational model of bone regeneration we show that particular cell patterns in tissue engineering constructs are able to enhance bone regeneration compared to uniform ones. We successfully bioprinted one of the most promising cell-gradient patterns by using cell-laden hydrogels with varying cell densities and observed a high cell viability for three days following the bioprinting process. In summary, we present a novel strategy for the biofabrication of bone tissue engineering constructs by designing cell-gradient patterns based on a computational model of bone regeneration, and successfully bioprinting the chosen design. This integrated approach may increase the success rate of implanted tissue engineering constructs for critical size bone defects and also can find a wider application in the biofabrication of other types of tissue engineering constructs.

Item Type:Article
Uncontrolled Keywords:bioprinting; cell-laden hydrogels; computational model; cell pattern; bone tissue engineering; non-healing bone defects
Subjects:T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
T Technology > TA Engineering (General). Civil engineering (General) > TA401-492 Materials of engineering and construction. Mechanics of materials
ID Code:30695
Deposited By:Bahattin Koç
Deposited On:08 Nov 2016 11:03
Last Modified:08 Nov 2016 11:03

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