title   
  

Single cell analysis on the role of TGF-β1 in autophagy of tumor cell-interacted stroma fibroblasts

Kim, Junyoung and Karakaş Schüller, Hacer Ezgi and Gözüaçık, Devrim and Cho, Yoon-Kyoung (2015) Single cell analysis on the role of TGF-β1 in autophagy of tumor cell-interacted stroma fibroblasts. In: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015), Gyeongju, South Korea

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Abstract

The communication between carcinoma associated fibroblast cells (CAFs) and tumor cells and autophagy activation mechanism during this interaction are important in the survival, proliferation and metastasis of cancer. Here we introduce a single cell-based screening platform consisted of PDMS porous membrane. In this paper, single cell-based screening platform is used (1) to trap single tumor cells by using gravitation force and agitation, (2) to monitor the autophagy of fibroblasts after interaction, and (3) to study paracrine factors from the heterogenic tumor cells inducing autophagy activation in fibroblasts. Our study highlights the role of TGF-β1 secreted by tumor cells in the autophagy activation in fibroblast using a novel screening system.

Item Type:Papers in Conference Proceedings
Uncontrolled Keywords:Autophagy; Fibroblast; Single cell-based screening; TGF-β1; Tumor
Subjects:Q Science > QH Natural history > QH506 Molecular biology
Q Science > Q Science (General)
R Medicine > R Medicine (General)
ID Code:29668
Deposited By:Devrim Gözüaçık
Deposited On:12 Nov 2016 21:22
Last Modified:12 Nov 2016 21:22

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