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Effect of codon optimization on the expression of trichoderma reesei endoglucanase 1 in pichia pastoris

Bayram Akçapınar, Günseli and Gül, Özgür and Sezerman, Uğur (2011) Effect of codon optimization on the expression of trichoderma reesei endoglucanase 1 in pichia pastoris. Biotechnology Progress, 27 (5). pp. 1257-1263. ISSN 8756-7938

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Official URL: http://dx.doi.org/10.1002/btpr.663

Abstract

Trichoderma reesei cellulases are important biocatalysts for a wide range of industrial applications that include the paper, feed, and textile industries. T. reesei endoglucanase 1 (egl1) was successfully expressed as an active and stable catalyst in Pichia pastoris for the first time. Codon optimization was applied to egl1 of T. reesei to enhance its expression levels in P. pastoris. When compared with the originally cloned egl1 gene of T. reesei, the synthetic codon optimized egl1 gene (egl1s) was expressed at a higher level in P. pastoris. Batch fermentations of both clones with the same copy number under controlled conditions indicated that codon optimized EGI enzyme activity increased to 1.24 fold after 72 h of methanol induction. Our research indicated that P. pastoris is a suitable host for cellulase production.

Item Type:Article
Uncontrolled Keywords:cellulose; endoglucanase 1; codon optimization; Trichoderma reesei; Pichia pastoris; recombinant expression
Subjects:T Technology > TP Chemical technology > TP0248.13 Biotechnology
Q Science > QR Microbiology
ID Code:17502
Deposited By:Uğur Sezerman
Deposited On:23 Nov 2011 15:31
Last Modified:08 Jan 2012 21:52

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