Virus induced gene silencing, a post transcriptional gene silencing method

Unver, Turgay and Budak, Hikmet (2009) Virus induced gene silencing, a post transcriptional gene silencing method. International Journal of Plant Genomics, 2009 . ISSN 1687-5370

[img]MS Word (This is a RoMEO green publisher -- author can archive pre-print (ie pre-refereeing) and post-print (ie final draft post-refereeing))

Official URL: http://dx.doi.org/10.1155/2009/198680


Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence-specific manner. Therefore, different types of methodologies have been advanced and modified for VIGS approach. Virus-derived inoculations are performed on host plants using different methods such as agro-infiltration and in vitro transcriptions. VIGS has many advantages compared to other loss-of-gene function approaches. The approach provides the generation of rapid phenotype and no need for plant transformation. The cost of VIGS experiment is relatively low, and large-scale analysis of screening studies can be achieved by the VIGS. However, there are still limitations of VIGS to be overcome. Nowadays, many virus-derived vectors are optimized to silence more than one host plant such as TRV-derived viral vectors which are used for Arabidopsis and Nicothiana benthamiana. By development of viral silencing systems monocot plants can also be targeted as silencing host in addition to dicotyledonous plants. For instance, Barley stripe mosaic virus (BSMV)-mediated VIGS allows silencing of barley and wheat genes. Here we summarize current protocols and recent modified viral systems to lead silencing of genes in different host species.

Item Type:Article
Subjects:Q Science > Q Science (General)
ID Code:13602
Deposited By:Hikmet Budak
Deposited On:22 Dec 2009 09:41
Last Modified:24 Jul 2019 14:40

Repository Staff Only: item control page