Ghaffari Zaki, Asal and Miri, Seyed Mohammad and Çimen, Şeyma and Akgül Çağlar, Tüba and Yiğit, Esra N. and Aydın, Mehmet Ş and Öztürk, Gürkan and Eroğlu, Emrah (2023) Development of a chemogenetic approach to manipulate intracellular pH. Journal of the American Chemical Society, 145 (22). pp. 11899-11902. ISSN 0002-7863 (Print) 1520-5126 (Online)
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Official URL: https://dx.doi.org/10.1021/jacs.3c00703
Abstract
Chemogenetic Operation of iNTRacellular prOton Levels (pH-Control) is a novel substrate-based enzymatic method that enables precise spatiotemporal control of ultralocal acidification in cultured cell lines and primary neurons. The genetically encoded biosensor SypHer3s showed that pH-Control effectively acidifies cytosolic, mitochondrial, and nuclear pH exclusively in the presence of β-chloro-d-alanine in living cells in a concentration-dependent manner. The pH-Control approach is promising for investigating the ultralocal pH imbalance associated with many diseases.
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Engineering and Natural Sciences > Academic programs > Biological Sciences & Bio Eng. Faculty of Engineering and Natural Sciences |
| Depositing User: | Emrah Eroğlu |
| Date Deposited: | 07 Aug 2023 12:20 |
| Last Modified: | 07 Aug 2023 12:20 |
| URI: | https://research.sabanciuniv.edu/id/eprint/47400 |
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