A new microfluidics system with a hand-operated, on-chip actuator for immunosensor applications

Kallempudi, Sreenivasa Saravan and Altıntaş, Zeynep and Kolkar Mohammed, Javed Hussain Niazi and Gürbüz, Yaşar (2012) A new microfluidics system with a hand-operated, on-chip actuator for immunosensor applications. Sensors and Actuators B: Chemical, 163 (1). pp. 194-201. ISSN 0925-4005

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This paper presents the realization of a portable, point-of-care and multi-target immunosensing lab-on-a-chip (LOC). The chip utilizes a novel on-chip actuating mechanism which uses a negative pressure to create thrust inside the channels to facilitate and control the fluid flow. No external instruments and/or sources were used to drive or control the liquid throughout the experimentation. LOC demonstrates a highly sensitive detection and quantification of a cancer marker, human-epidermal growth factor receptor (hEGFR) and a cardiac marker, interleukin 6 (IL-6) by impedimetric measurement approach. Without the use of any signal amplification methods (chemical), a dynamic detection limit of 3-8 ng/ml of hEGFR and 0.1-5 ng/ml of IL-6 in human serum was obtained. The extracted dissociation constants were calculated as 5.86 ng/ml (hEGFR) and 2.45 ng/ml (IL-6) by kinetic analysis.
Item Type: Article
Uncontrolled Keywords: Biosensor; Immunosensor; Cancer marker detection; Cardiovascular disease marker detection; Lab-on-a-Chip
Subjects: R Medicine > R Medicine (General) > R856-857 Biomedical engineering. Electronics. Instrumentation
Q Science > QD Chemistry
Divisions: Faculty of Engineering and Natural Sciences > Academic programs > Electronics
Faculty of Engineering and Natural Sciences
Depositing User: Yaşar Gürbüz
Date Deposited: 06 Apr 2012 11:55
Last Modified: 31 Jul 2019 10:47
URI: https://research.sabanciuniv.edu/id/eprint/18974

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