Adsorption of bovine serum albumin (BSA) on clinoptilolite

Abstract

The purpose of the present work was to investigate the adsorption of BSA onto the clinoptilolite in aqueous media. In this study, batch adsorption experiments were carried out and the effects of pH, protein concentration, ionic strength and Si/Al ratio of zeolite on the adsorption process were examined. The optimum pH for adsorption was found to be 4.0. It was found that treatments applied to modify clinoptilolite had substantial effects on the adsorption. During adsorption , residual BSA concentration reached equilibrium in 60 min. Maximum adsorption capacity value for the raw clinoptilolite was determined as 388.3 mg BSA / g zeolite (23.2 mg BSA/m2) and for the treated clinoptilolite, was ranged between 500-660 mg BSA/g zeolite (12.8-31.8 mgBSA/ m2).