title   
  

Sweet cherry cultivar identification by using SSR markers

Aka Kaçar, Yıldız and Lezzoni, Amy F. and Çetiner, Selim (2005) Sweet cherry cultivar identification by using SSR markers. Journal of Biological Sciences, 5 (5). pp. 616-619. ISSN 1727-3048 (print) 1812-5719 (online)

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Official URL: http://dx.doi.org/10.3923/jbs.2005.616.619

Abstract

In this study, sweet cherry varieties and types grown in Turkey were described using SSR markers. This study was undertaken to develop DNA marker profiles that could be used to distinguish among the sweet cherry cultivars used in production in Turkey. For microsatellite analysis, 13SSR primers isolated from sweet cherry (P. avium L.) sour cherry (P. cerasus L.) and peach (P. persica L. Batsch) were used on sweet cherry cultivars and types. Two primer pairs did not give amplification with genotypes analyzed. Two-primer pairs amplified monomorphic fragment for the sweet cherry varieties therefore they were uninformative for the sweet cherry genetic analysis. Genetic similarities were calculated and a dendrogram has been established. All of the 8 SSR primers used and cherries have produced amplified bands. For each primer the alleles obtained has been between 1 and 6, in total 38 alleles have found. Through these analyses the similarities between these varieties have been converted into numerical values. This happens to be the first study kind towards the molecular identification of sweet cherry genetic resources in Turkey

Item Type:Article
Subjects:Q Science > Q Science (General)
S Agriculture > SB Plant culture
ID Code:618
Deposited By:Selim Çetiner
Deposited On:16 Oct 2005 03:00
Last Modified:11 Jan 2010 15:11

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